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Name: 4-Hydroxyethylpiperazineethanesulfonic acid (HEPES)

Synonyms: 

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;

4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid;

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; 

N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid);

1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-;

2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid;

2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid.

CAS No.: 7365-45-9

EINECS: 230-907-9

MF: C8H18N2O4S

MW: 238.30

MP: 234-238 °C

BP: N/A

FP: N/A

Density: 1.3±0.1 g/cm3

Index of Refraction: 1.543

Molar Refractivity: 56.7±0.4 cm3

Purity: 97% Min.

Appearance: White powder

Application: Biological buffering agent; Can be used in cosmetics

Storage Condition: Store in a cool, dry place. Keep the container closed when not in use.

HEPES is an important hydrogen ion buffer, which has a good buffering capacity in the range of pH 6.8-8.2 and can control constant pH for a long time. The concentration used is 10-50 mmol/L. Generally, the culture medium contains 20 mmol/L of HEPES, which can have a good buffering ability and has no toxic effect on cells.

HEPES is a non-ionic amphoteric buffer, which is highly polar, inert to a variety of chemical reagents and enzymes, does not participate in and does not interfere with the process of biochemical reactions, and has no inhibitory effect on enzymatic chemical reactions, so it can be specially used for Research work on organelles and highly labile, pH-sensitive proteins and enzymes.

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